Isolate DNA from a plant tissue (LC 2026) (Leaving Cert Biology): Flashcards
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Practise the cards
13 cards from this deck
Purpose of chopping kiwi in DNA isolation
Purpose of chopping kiwi in DNA isolation
Breaks down cell wall & increases surface area
Role of detergent in DNA isolation
Role of detergent in DNA isolation
Dissolves cell membranes
Role of salt in DNA isolation
Role of salt in DNA isolation
Helps DNA to clump together
Water bath temp for DNA isolation
Water bath temp for DNA isolation
60°C
Time in water bath at 60°C
Time in water bath at 60°C
15 minutes
Why heat at 60°C in DNA isolation?
Why heat at 60°C in DNA isolation?
Denatures enzymes that would break down DNA
Time in ice-water bath during DNA isolation
Time in ice-water bath during DNA isolation
5 minutes
Why cool in ice-water bath?
Why cool in ice-water bath?
Stops breakdown of DNA
Max blending time in DNA isolation
Max blending time in DNA isolation
3 seconds
Why blend for no more than 3 seconds?
Why blend for no more than 3 seconds?
To avoid DNA degradation
Amount of protease to add
Amount of protease to add
2-3 drops
DNA solubility in cold ethanol
DNA solubility in cold ethanol
Insoluble
Where DNA appears when ethanol added
Where DNA appears when ethanol added
At ethanol-filtrate boundary