Preparation and use of an immobilised enzyme (LC 2026) (Leaving Cert Biology): Flashcards
📚Flashcards
Practise the cards
12 cards from this deck
ShowHide
Practise the cards
12 cards from this deck
What are immobilised enzymes?
What are immobilised enzymes?
Enzymes attached to an inert material
Material mixed with water and yeast to form paste
Material mixed with water and yeast to form paste
Sodium alginate
Solution beads are dropped into
Solution beads are dropped into
Calcium chloride solution
Why sterilise glassware before the experiment?
Why sterilise glassware before the experiment?
To prevent contamination from microorganisms
How is mixture released into calcium chloride?
How is mixture released into calcium chloride?
One drop at a time
What forms in calcium chloride solution?
What forms in calcium chloride solution?
Beads containing yeast cells
Why rinse beads with distilled water?
Why rinse beads with distilled water?
To remove excess free enzyme
Product made from sucrose in this experiment
Product made from sucrose in this experiment
Glucose
Substrate added to separating funnels
Substrate added to separating funnels
Sucrose solution
Test used to detect glucose
Test used to detect glucose
Clinistix
Property compared between free and immobilised yeast
Property compared between free and immobilised yeast
Turbidity of the solutions
Three materials mixed to form paste
Three materials mixed to form paste
Sodium alginate, water, and yeast