Grow a Bacteria Culture (Leaving Cert Agricultural Science): Flashcards

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Grow a Bacteria Culture
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What does nutrient agar provide?

Food for bacterial growth

What do methylated spirits sterilise?

The inoculating loop

Incubation temperature for bacteria culture

30°C30°C

Incubation time for bacteria culture

24 hours

Loop cooling time after sterilising

30 seconds

Angle to open petri dish during inoculation

45°45° angle

Purpose of control plate (Plate B)

Verify growth came from soil sample, not contamination

Why are plates placed upside-down in incubator?

Prevents condensation dripping onto agar surface

Bacterial colonies appearance

Small, round, often coloured spots

Fungal colonies appearance

Larger, fuzzy or cotton-like texture

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