Isolate & Grow Bacteria (Leaving Cert Agricultural Science): Quizzes

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Isolate & Grow Bacteria
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What type of bacteria is isolated from clover root nodules?

Rhizobium bacteria

What colour are active root nodules on legume plants?

Pink or reddish

What protein causes the pink colour in root nodules?

Leg-haemoglobin

Rhizobium converts atmospheric nitrogen (N2\text{N}_2) into which compound?

Ammonia (NH3\text{NH}_3)

What % of nitrogen needs can legumes meet through biological nitrogen fixation?

Up to 80%

What concentration of ethanol is used to sterilise nodules?

70% ethanol

At what temperature should the Petri dishes be incubated?

25-28°C

What is the correct method sequence for isolating Rhizobium?

Sterilise → crush → streak → incubate

What appearance indicates successful Rhizobium isolation?

Small, white, circular colonies

What is the main agricultural benefit of the Rhizobium-legume relationship?

Reduces need for nitrogen fertilisers

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