Isolate & Grow Bacteria (Leaving Cert Agricultural Science): Flashcards

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Isolate & Grow Bacteria
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Name of bacteria isolated from clover nodules

Rhizobium bacteria

Location of Rhizobium on legume roots

Root nodules

Process demonstrated by Rhizobium

Nitrogen fixation

What Rhizobium converts N2\text{N}_2 into

Ammonia (NH3\text{NH}_3)

Colour of active root nodules

Pink or reddish

Protein causing pink colour in nodules

Leg-haemoglobin

Incubation temperature for Rhizobium

25-28°C

Expected Rhizobium colony appearance

Small, white, circular

Four main steps of isolation method

Sterilise → crush → streak → incubate

Agricultural benefit of N fixation

Reduces need for artificial fertilisers

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