The Presence of Lactose in Milk (Leaving Cert Agricultural Science): Flashcards

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The Presence of Lactose in Milk
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Lactose classification

Reducing sugar

Lactose % in cow's milk

4-5%

Benedict's test detects

Reducing sugars

Benedict's test: colour change

Blue to brick-red

Benedict's test: temperature

80-100°C

Benedict's test: heating time

5 minutes

Why lactose is a reducing sugar

Has free anomeric carbon

Purpose of water control in test

Shows no reducing sugars present, proves specificity

Positive Benedict's test shows

Colour changes from blue to brick-red/orange

Chemical change in Benedict's test

Cu2+Cu^{2+} (blue) reduced to Cu2OCu_2O (brick-red)

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