Exploring DNA Technology (VCE SSCE Biology): Flashcards
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Practise the cards
10 cards from this deck
DNA manipulation definition
DNA manipulation definition
Lab techniques to work with genetic material
3 fundamental DNA techniques
3 fundamental DNA techniques
DNA extraction, PCR, gel electrophoresis
DNA extraction purpose
DNA extraction purpose
Isolating DNA molecules from cells
Role of alcohol in DNA extraction
Role of alcohol in DNA extraction
Causes DNA to precipitate & form visible white strands
What PCR does
What PCR does
Amplifies specific DNA segments, creating millions of copies
PCR denaturing temperature
PCR denaturing temperature
Why Taq polymerase used in PCR
Why Taq polymerase used in PCR
Can withstand high temps unlike human DNA polymerase
What gel electrophoresis separates
What gel electrophoresis separates
DNA fragments by size and electrical charge
DNA charge & migration in gel
DNA charge & migration in gel
Negatively charged; migrates towards positive electrode
DNA fragment size vs distance in gel
DNA fragment size vs distance in gel
Smaller fragments travel further through gel