Extracting DNA From Strawberries (v2) (VCE SSCE Biology): Quizzes

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Extracting DNA From Strawberries (v2)
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What does it mean that strawberries are octoploid?

They have 8 copies of each chromosome

Why does DNA precipitate when isopropyl alcohol is added?

DNA is soluble in water but not alcohol

What is the role of detergent in DNA extraction?

Breaks down fatty cell membranes

What is the white, stringy material observed after adding alcohol?

Thousands of DNA molecules clumped together

What role does salt play in DNA extraction?

Helps DNA stick together and clump

What is a lysis buffer?

A solution that breaks open cell membranes

What are the four main stages of DNA extraction in order?

Mashing, lysis buffer, filtering, alcohol

What is the filtrate in this experiment?

Liquid that passes through filter paper

Which enzymes naturally present in strawberries help break down cell walls?

Pectinases and cellulases

What is the purpose of adding isopropyl alcohol to the filtrate?

To make DNA precipitate and become visible

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