Exploring DNA Technology (VCE SSCE Biology): Quizzes

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Exploring DNA Technology
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What are the three fundamental DNA manipulation techniques?

DNA extraction, PCR, gel electrophoresis

What is the primary purpose of DNA extraction?

Isolating DNA molecules from cells

What role does salt solution play in DNA extraction?

Neutralizes negative charges on DNA

What does alcohol do during DNA extraction?

Causes DNA to precipitate as white strands

What happens to target DNA during each PCR cycle?

Amount doubles

What temperature is used for the denaturing stage of PCR?

9495°C94-95°C

Why is Taq polymerase essential for PCR?

It can withstand high temperatures

What is the function of primers in PCR?

Mark start and end of DNA region to copy

Why does DNA migrate toward the positive electrode in gel electrophoresis?

DNA is negatively charged

How does fragment size relate to migration distance in gel electrophoresis?

Smaller fragments travel further

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