Extracting DNA From Strawberries (VCE SSCE Biology): Flashcards
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DNA full form
DNA full form
Deoxyribonucleic acid
Genome definition
Genome definition
The entire set of genetic instructions
Why strawberries ideal for DNA extraction
Why strawberries ideal for DNA extraction
Octoploid (8 copies) - yields more DNA than most fruits
Enzymes in strawberries aiding extraction
Enzymes in strawberries aiding extraction
Pectinases and cellulases
Lysis buffer purpose
Lysis buffer purpose
Breaks down cell membranes and releases DNA
Lysate definition
Lysate definition
Mixture containing broken-down cells
Salt role in lysis buffer
Salt role in lysis buffer
Helps DNA strands stick together, separates from proteins
Detergent role in lysis buffer
Detergent role in lysis buffer
Breaks down lipid membranes of cells and nuclei
DNA solubility: water vs alcohol
DNA solubility: water vs alcohol
Soluble in water, not in alcohol (precipitates)
4 main DNA extraction steps
4 main DNA extraction steps
Mash → Lyse → Filter → Precipitate (MLFO)