Observing Mitosis in Onion Root Cells (VCE SSCE Biology): Flashcards

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Observing Mitosis in Onion Root Cells
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Why onion root cells ideal for mitosis observation

Constantly dividing & growing

Four phases of mitosis

Prophase, Metaphase, Anaphase, Telophase

Purpose of HCl & heat treatment in mitosis practical

Breaks down cell walls/pectin

Water bath temperature for onion root cells

60°C

What Feulgen stain specifically binds to

DNA

Magnification to observe onion root mitosis

400×

More cells in a stage means

That stage takes longer to complete

Mitosis stage with typically highest % of cells

Prophase

Mitosis stage with typically fewest cells

Anaphase

Formula: % of cells at a stage

cells at stagetotal cells×100\frac{\text{cells at stage}}{\text{total cells}} \times 100

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